Abstract
Current assays for screening new antimalarials need initiators of beta-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in beta-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of beta-hematin formation.
MeSH terms
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Animals
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Antimalarials / pharmacology*
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Colorimetry / methods
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Crystallization
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Drug Evaluation, Preclinical / methods
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Heme / chemistry
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Heme / metabolism*
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Hemeproteins / antagonists & inhibitors
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Hemeproteins / metabolism
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Plasmodium falciparum / drug effects*
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Plasmodium falciparum / metabolism
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Polysorbates / chemistry
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Reproducibility of Results
Substances
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Antimalarials
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Hemeproteins
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Polysorbates
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Heme